Development and Optimisation of Hydrogel Scaffolds for Microvascular Network Formation

Traumatic injuries are a major cause of morbidity and mortality worldwide; however, there is limited research on microvascular traumatic injuries. To address this gap, aims to develop optimise an in vitro construct for injury at the level. Tissue engineering constructs were created using range polymers (collagen, fibrin, gelatine), solvents (PBS, serum-free endothelial media, MES/NaCl buffer), concentrations (1–5% w/v). Constructs from these hydrogels HUVECs evaluated identify optimal composition terms cell proliferation, adhesion, migration rate, viability, hydrogel consistency shape retention, tube formation. Gelatine associated with lower whereas fibrin collagen ones displayed similar or better results than control, exhibited poor retention; scaffolds, particularly high concentrations, good consistency. Based multipronged evaluation, media 3 5% w/v selected further experimental work enabled formation interconnected capillary-like networks. The networks formed both architecture number segments (10.3 ± 3.21 vs. 9.6 3.51) diameter (8.6446 3.0792 μm 7.8599 2.3794 μm).